TABLE 3.

Fatty acid contents and compositions of fatty acids of A. borkumensis SK2 and transposon-induced mutants^a

Strain	Median biomass (g/liter) ± SD	Total TAG content (% CDW)	Amt (mol%) of TAG constituent:
			C14:0	C16:0	C16:1	C18:0	C18:1
SK2	2.7 ± 0.42	13	4	41	17	3	35
atfA1 atfA2 mutant	2.55 ± 0.07	3	0	45	16	0	39
2 M21	1.02 ± 0.28	8	2	42	19	0	36
2 M61	1.34 ± 0.25	6	3	42	20	0	35
2 M91	2.51 ± 0.55	3	0	42	19	4	36
2 M93	0.79 ± 0.38	7	0	41	22	0	37
2 M111	1.31 ± 0.35	4	0	42	21	0	37
2 M131	2.05 ± 0.21	ND	ND	ND	ND	ND	ND
2 M141	1.07 ± 0.13	6	3	40	19	2	37
2 M191	2.44 ± 0.06	14	4	34	8	41	12
2 M192	2.24 ± 0.06	4	0	34	17	18	31
2 M193	1.08 ± 0.06	10	2	37	19	3	38
2 M231	2.7 ± 0.17	4	0	43	21	0	37
2 M232	1.91 ± 0.16	5	0	41	18	3	39
2 M233	2.56 ± 0.17	3	0	42	20	0	38

^aCells were cultivated in ONR7a medium containing 1% (wt/vol) sodium pyruvate for 72 h at 30°C. Lipid extracts were obtained from 5-mg samples of lyophilized cells (see the text for details). TAGs were purified by preparative TLC before GC analysis. Tridecanoic acid was used as an internal standard for quantification and was added to the samples before the start of acid-catalyzed methanolysis. For the molar compositions of fatty acids, no trace amounts (<1 mol%) were considered. The values are the averages of two independent determinations. SK2, A. borkumensis wild type; atfA1 atfA2 mutant, A. borkumensis atfA1ΩKm atfA2ΩSm; ND, not determined. 2 M designations denote transposon-induced mutants.