TABLE 1.
Stress responses of WT and R clonesa
| Strain | Stress response to: |
|||
|---|---|---|---|---|
| Bile salt (0.3%) | H2O2 (4 mM) | H2O2 (10 mM) | pHb | |
| 23K | 0.90 ± 0.10 | 3.20 ± 0.30 | >5 | <0.4 |
| 23K-J10SN2 | 2.10 ± 0.90 | 3.80 ± 0.70 | >5 | ND |
| 23K-J30RO2 | 3.90 ± 0.70 | 5.00 ± 0.20 | >5 | 2.4 ± 0.20c |
| FLEC01 | 5.20 ± 0.00 | 4.05 ± 0.15 | >5 | <0.4 |
| FLEC01-J6SN2 | 4.10 ± 0.10 | 3.40 ± 0.20 | >5 | ND |
| FLEC01-J15RN2 | 3.95 ± 0.15 | 3.40 ± 0.10 | >5 | <0.4 |
| LTH5590 | 5.35 ± 0.05 | >5 | >5 | <0.4 |
| LTH5590-J21SO2 | 3.50 ± 0.10 | 3.80 ± 0.50 | 4.10 ± 0.20 | ND |
| LTH5590-J40RN2 | 1.75 ± 0.15 | 0.60 ± 0.10 | 2.27 ± 1.0 | <0.4 |
a
Stress response is expressed as the loss of cell viability (Δlog CFU/ml) after the various stress conditions tested, as described in Materials and Methods.
b
Values were similar for pH values ranging from 3 to 6.0. ND, not determined.
c
Value is given for pH 3.