TABLE 3.

Cytosolic and cell wall/membrane-associated proteins downregulated in R clone L. sakei 23K-J30RO2^a

Protein type	Functional category	Function	Locus tag	ANOVAa	Fold vol change
Cytoplasm	Adaptation to atypical conditions	Putative general stress protein	LSA0169	0.003	34
		Putative general stress protein	LSA0170	0.017	6.2
		Universal stress protein (Usp5)	LSA0038	0.030	4.3
	Oxidoreduction	Putative chromate reductase (flavoprotein)	LSA1317	0.002	8.5
		Organic hydroperoxide resistance protein (OhrA)	LSA0552	0.030	6.5
		Putative oxidoreductase, short-chain dehydrogenase/reductase family	LSA0165	0.005	2.7
	Carbohydrate metabolism	Pyruvate dehydrogenase complex, E1 component, beta subunit (PdhB)	LSA1084	0.041	3.2
	Amino acid metabolism	Putative 2-hydroxyacid dehydrogenase	LSA0463	0.003	3.2
	Transcription-translation	30S ribosomal interface protein S30EA	LSA0494	0.007	4.0
Cell wall/membrane	Adaptation to atypical conditions	Universal stress protein (Usp6)	LSA0836	0.004	2.1
	Transport and binding	Oligopeptide ABC transporter, ATP-binding subunit (OppD)	LSA0705	0.022	3.5
		Oligopeptide ABC transporter, ATP-binding subunit (OppF)	LSA0706	0.007	3.4
		Putative drug ABC exporter, ATP-binding subunit	LSA0938	0.026	3.3
		Putative glycine/betaine/carnitine/choline ABC transporter, ATP-binding subunit	LSA0616	0.023	3.1
	Oxidoreduction	Thiol peroxidase (Tpx)	LSA0104	0.003	5.3
		Putative aldo/keto reductase (oxidoreductase)	LSA0030	0.001	2.6
		Putative nitroreductase (oxidoreductase)	LSA1712	0.001	2.5
	Protein modification	ATPase/chaperone (ClpC)	LSA1779	0.023	2.6
	Amino acid metabolism	Glycine/serine hydroxymethyltransferase (GlyA)	LSA1134	0.037	2.7
		Putative aminopeptidase	LSA1426	0.002	3.1
	Transcription-translation	30S ribosomal interface protein S30EA	LSA0494	0.004	3.7
	RNA restriction modification	Putative RNA methyltransferase	LSA1544	0.012	1.9
Surface exposed	Adaptation to atypical conditions	Universal stress protein (Usp6)	LSA0836	0.004	2.1
	Transport and binding	Putative glycine/betaine/carnitine/choline ABC transporter, ATP-binding subunit	LSA0616	0.0006	3.9
		Oligopeptide ABC transporter, ATP-binding subunit (OppD)	LSA0705	0.030	2.4
		Oligopeptide ABC transporter, ATP-binding subunit (OppF)	LSA0706	0.004	2.3
		Putative ABC exporter, ATP-binding subunit	LSA1366	0.046	2.1
	Oxidoreduction	Thiol peroxidase (Tpx)	LSA0104	0.037	2.4
	Carbohydrate metabolism	Pyruvate oxidase (Pox1)	LSA1188	0.012	2.3
		Pyruvate kinase (Pyk)	LSA1032	0.033	1.7

^aStatistical validation was performed by the use of an ANOVA test provided by SameSpots (see Materials and Methods).