TABLE 2.

Summary of experimental conditions and disinfectant decay parameters used for assessing the inactivation of adenovirus serotype 2 with free chlorine^a

Expt	pH	N0 (PFU/ml)	c0 (mg Cl2/liter)	c0*b (mg Cl2/liter)	kd (min−1)	Capsid protein integrity (H/H0, F/F0)	CAR affinity (C/C0)	Attachment in cell culture (A/A0)	Genome/DNA integrity (D/D0)	E1A synthesis ability (E/E0)
1	7.4	4.6 × 106	1.48	1.48	114			X	X
2	7.4	1.4 × 106	1.75	1.75	111			X	X
3	7.4	1.6 × 106	1.90	1.90	108			X	X
4	7.4	4.2 × 106	1.90	1.82	101					X
5	7.4	6.7 × 106	4.00	4.00	276	X				X
6	7.4	2.2 × 107	4.08	3.69	235					X
7	7.4	3.4 × 107	3.67	3.07	356					X
8	7.4	1.1 × 108	6.37	5.21	271	X	X
9	7.4	1.3 × 108	5.16	4.48	266	X	X
10	7.4	1.5 × 108	5.16	3.88	255	X	X
11	7.4	1.0 × 107	1.25	0.73	0.919	X
12	10.0	3.9 × 106	1.31	0.85	0.087			X	X
13c	10.0	9.0 × 108	2.11	1.24	2.17					X
			0.25	0.24	1.82
			0.50	0.51	0.260
14	10.0	1.1 × 108	3.70	1.54	0.687					X
15	10.0	1.2 × 108	4.24	2.04	0.713					X
16	10.0	4.2 × 107	3.94	1.15	0.633	X	X	X	X	X
17	10.0	8.0 × 107	3.12	1.30	0.461			X	X	X
18d	10.0	1.4 × 109	12.3	5.46	4.04	X	X
			6.50	6.44	0.957
19	10.0	1.0 × 107	5.32	1.81	0.272	X

^aThe experimental conditions used for the inactivation of adenovirus serotype 2 with free chlorine were the pH and the initial concentrations of viable virions (N₀) and disinfectant (c₀), and the disinfectant decay parameters were the decomposition rate constant (k_d) and the extrapolated initial disinfectant concentration (c₀*). X, virus samples from the given experiment were subjected to the corresponding molecular analysis (D/D₀, E/E₀, A/A₀, H/H₀, F/F₀, and C/C₀ are normalized parameters for the various molecular signals defined in Results).

^bc₀*, modified initial free chlorine concentration obtained by extrapolation of the chlorine data to 0 min (or the time of midexperiment dosing for experiments 13 and 18) using a first-order decay model as described previously (19).

^cChlorine was dosed three times during the performance of experiment 13, i.e., c_1,0 = 2.11 mg Cl₂/liter at 0 min, c_2,0 = 0.25 mg Cl₂/liter at 2 min, and c_3,0 = 0.50 mg Cl₂/liter at 3 min.

^dChlorine was dosed twice during the performance of experiment 18, i.e., c_1,0 = 12.3 mg Cl₂/liter at 0 min and c_2,0 = 6.50 mg Cl₂/liter at 1.5 min.