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. 2010 Mar 5;76(9):2704–2711. doi: 10.1128/AEM.02979-09

FIG. 2.

FIG. 2.

Isoelectric focusing and CAS overlay for detection of siderophores in P. syringae. Siderophores were extracted from concentrated culture supernatants using XAD-4 resin at pH 3.0. After separation by isoelectric focusing, the polyacrylamide gel was overlaid with a thin layer of CAS agar to visualize siderophore activity. Lane 1, P. syringae pv. syringae 22d/93ΔSid; lane 2, purified pyoverdin of P. syringae; lane 3, P. syringae pv. syringae 22d/93; lane 4, P. syringae pv. glycinea 1a/96; lane 5, P. syringae pv. syringae 22d/93ΔPvd.