FIG. 3.

Regulation of CYP gene expression in Symbiodinium symbiotic with a coral host (A. millepora) exposed to rapid and gradual thermal stress regimens with a maximum temperature of 32°C. The relative quantities were normalized using data for two of the most stable reference genes defined by the GeNorm analysis, the Rp-S4 and SAM genes (M = 0.351) with pairwise variations for V2/3 of 0.137. The ratio of the CYP gene expression for the treated sample to the CYP gene expression for the control at each time point (T:CTRL) is shown for CYP-1 (A), CYP-2 (B), and CYP-3 (C). All data are the means ± standard deviations of values obtained for four independent biological replicates.