TABLE 2.
Seeds | Assay | No. of seed samples | Multiplex nested PCR detection |
Semiselective isolation |
No. of lots detected by both methods | ||
---|---|---|---|---|---|---|---|
CR (%) | No. of positive lots | CR (%) | No. of positive lots | ||||
Seed lot P2 (CR, 1/10,000)a | Assay 1 | 35 | 0.000077 | 6 | 0.000050 | 4 | 3 |
Assay 2 | 35 | 0.000121 | 9 | 0.000012 | 1 | 1 | |
Mean | 0.000099 | 0.000031 | |||||
Seed lot P2 mixed with healthy seeds (1:1.1) (CR, 1/21,000)b | Assay 1 | 35 | 0.000050 | 4 | 0.000000 | 0 | 0 |
Assay 2 | 35 | 0.000037 | 3 | 0.000024 | 2 | 0 | |
Mean | 0.000043 | 0.000012 | |||||
Seed lot P2 mixed with healthy seeds (1:2) (CR, 1/30,000)b | Assay 1 | 35 | 0.000037 | 3 | 0.000000 | 0 | 0 |
Assay 2 | 35 | 0.000037 | 3 | 0,000012 | 1 | 0 | |
Mean | 0.000037 | 0.000006 | |||||
Seed lot P2 mixed with healthy seeds (1:12.8) (CR, 1/138.000)b | Assay 1 | 35 | 0.000000 | 0 | 0.000012 | 1 | 0 |
Assay 2 | 35 | 0.000000 | 0 | 0.000000 | 0 | 0 | |
Assay 3 | 35 | 0.000037 | 3 | 0.000012 | 1 | 0 | |
Mean | 0.000012 | 0.000008 | |||||
Healthy seeds | Assay 1 | 35 | 0.000000 | 0 | 0.000000 | 0 | |
Assay 2 | 35 | 0.000000 | 0 | 0.000000 | 0 |
The seed contamination rate was determined by multiplex nested PCR analyses.
The theoretical seed contamination rate was obtained by diluting seed lot P2 with healthy seeds.