FIG. 2.

NOS-like activities (A) and intracellular cGMP levels (B) in mycelial extracts from five pycnidial development stages of C. minitans wild-type strain ZS-1. (A) Putative NOS-like activity was detected by measuring the conversion rate of l-[¹⁴C]citrulline from l-[¹⁴C]arginine. Values are means ± the standard error of four independent experiments. Bars with the same letter are not significantly different (P < 0.05). (B) Intracellular cGMP levels on PDA and on PDA amended with ODQ were estimated by using a ¹²⁵I-based radioimmunoassay. Values are means of four independent experiments. Bars represent the standard deviation from the mean (n = 4).