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. 2010 Feb 26;76(9):3039–3043. doi: 10.1128/AEM.02426-09

FIG. 3.

FIG. 3.

(A) Time-dependent antibiotic production in modified R5 plate cultures of S. coelicolor ΔwblA mutant (kindly provided by K. Chater) strains harboring pSE34 alone (left), pSE34 containing wblA (middle), and pSE34 containing SCO1712 (right). (B) Time-dependent antibiotic production in modified R5 plate cultures of wild-type S. coelicolor M145 (top), S. coelicolor ΔwblA (lower left), and S. coelicolor ΔwblAΔSCO1712 (lower right). The method for a PCR-targeted gene replacement disruption of SCO1712 in S. coelicolor ΔwblA was identical to those shown in Fig. 1 (data not shown). (C and D) Time-dependent growth curve (C) and ACT volumetric productivities (D) of wild-type S. coelicolor M145, S. coelicolor ΔwblA, and S. coelicolor ΔwblAΔSCO1712 cultured in modified R5 medium for 9 days in a 2-liter bioreactor (17). The averages of two independent fermentation experiments are shown with error bars.