TABLE 1.
Oligonucleotide primers designed and used in this study for SYBR green qPCR
| Target gene | Primer | Model gene(s) used for primer design | Sequence (5′→3′) | Product length (bp)a |
|---|---|---|---|---|
| rdpA | rdpA_f | 16 known rdpA sequences in GenBank | CACTTACCAGGTCATCTA | |
| rdpA_r | GTGTACATGCTCAGAAAAC | 285 | ||
| sdpA | sdpA_f | D. acidovorans MC1, S. herbicidovorans MH, Rhodoferax sp. P230 | ATGCCGACAGCACCTACAT | |
| sdpA_r1 | D. acidovorans MC1 | GGTCTCGGGATGCACCTT | 287 | |
| sdpA_r2 | S. herbicidovorans MH, Rhodoferax sp. P230 | GGTCTCGGGATGGATCTT | 290 |
a
PCR product lengths for different combinations of forward and reverse primers are indicated. Primer sdpA_f in combination with sdpA_r1 produces a 287-bp product while sdpA_f in combination with sdpA_r2 produces a 290-bp product.