FIG. 9.

Immunolocalization of Acp on C. perfringens strain 13 during exponential growth phase by indirect immunofluorescence. Bar, 10 μM. Red staining fluorescence is due to the monomeric cyanin nucleic acid stain To-Pro-3, and green fluorescence is due to the secondary anti-mouse IgG coupled with Alexa Fluor 488 fluorophore. (A) C. perfringens strain 13 stained with depleted anti-Acp immune serum. (B) C. perfringens strain 13 stained with depleted anti-Acp preimmune serum as a control. (C) C. perfringens strain 13 acp::erm stained with depleted anti-Acp immune serum.