FIG. 5.

Complementation of E. coli MC1000 ΔnanT by T. forsythia nanT. (A) E. coli MC1000 ΔnanT was transformed with either pBAD18 or derivatives containing the nanT gene from T. forsythia (pBAD18-nanT-TF:pBAD-TF) or E. coli (pBAD18-nanT-coli:pBAD-EC), and growth was assessed on M9 agar plates containing (0.5%; 15 mM) sialic acid. All strains showed identical growth with glucose (0.5%) as the carbon source (data not shown). (B) The same strains were grown in M9 liquid medium, and growth was monitored by measuring the A₆₀₀ at the time points indicated. Experiments were repeated on four separate occasions with essentially identical results. WT, wild type.