TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Reference or source |
|---|---|
| Strains | |
| T. forsythia 43037 | W. Wade (Kings College London, UK) |
| E. coli | |
| MG1655 | 2 |
| MG1655 nanR | |
| (amber mutation) ΔnanC | I. Blomfield (University of Kent, UK) |
| MC1000 ompR::Tn10(tet) | I. Blomfield (University of Kent, UK) |
| MG1655 nanR | |
| (amber mutation) ΔnanC ompR::Tn10(tet) | This study |
| MC1000 | 6 |
| MC1000 ΔnanT::FRT::Km::FRT | This study |
| TOP10 | Invitrogen |
| Plasmids | |
| pCR2.1-TOPO | Invitrogen |
| pBAD18 | 14 |
| pKD13 | 9 |
| pKD46 | 9 |
| pBAD18-nanT-coli | This study |
| pBAD18-nanT-TF | This study |
| pCR2.1-TOPO-TF0033-34 | This study |