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SDS-PAGE of SEC-LS fractionation of PGH. Samples (20 μl) of odd-numbered SEC fractions (25 to 49) spanning the peaks containing PGH activity in Fig. 5 (elution volumes, 9.5 to 13.7) were applied to a 7.5% polyacrylamide gel and stained as described in Materials and Methods. Molecular masses (MM) in kilodaltons are indicated.
