TABLE 2.
Substrate | Activity (μM)a |
||
---|---|---|---|
NH2-terminal amino acid | COOH-terminal amino acid | GLU | |
Dipeptide | |||
Ala-Gln | ND | ND | |
Asp-Glu | ND | ND | |
Glu-Gln | ND | ND | |
Gly-Glu | 36 ± 7.5 | 42 ± 3.7 | |
Phe-Ala | ND | ND | |
Phe-Val | ND | ND | |
Pro-Gly | ND | ND | |
Pro-Leu | ND | ND | |
Ser-Glu | 114 ± 8 | 117 ± 18 | |
Thr-Glu | ND | ND | |
Trp-Gly | ND | ND | |
Tyr-Ala | ND | ND | |
Tyr-Leu | ND | ND | |
Val-Gln | ND | ND | |
Folate derivatives | |||
PABA-GLU | 1,072 ± 15.8 | ||
Folate | 25 ± 11.3 |
Substrate (1 mM) was incubated with purified PGH (3.2 μg), heat inactivated after incubation for 15 min at 37°C, and then analyzed as described in Materials and Methods. ND, not detectable. Average values (±SE) are given.