TABLE 2.
Activities of PGH with selected substrates
| Substrate | Activity (μM)a |
||
|---|---|---|---|
| NH2-terminal amino acid | COOH-terminal amino acid | GLU | |
| Dipeptide | |||
| Ala-Gln | ND | ND | |
| Asp-Glu | ND | ND | |
| Glu-Gln | ND | ND | |
| Gly-Glu | 36 ± 7.5 | 42 ± 3.7 | |
| Phe-Ala | ND | ND | |
| Phe-Val | ND | ND | |
| Pro-Gly | ND | ND | |
| Pro-Leu | ND | ND | |
| Ser-Glu | 114 ± 8 | 117 ± 18 | |
| Thr-Glu | ND | ND | |
| Trp-Gly | ND | ND | |
| Tyr-Ala | ND | ND | |
| Tyr-Leu | ND | ND | |
| Val-Gln | ND | ND | |
| Folate derivatives | |||
| PABA-GLU | 1,072 ± 15.8 | ||
| Folate | 25 ± 11.3 | ||
a
Substrate (1 mM) was incubated with purified PGH (3.2 μg), heat inactivated after incubation for 15 min at 37°C, and then analyzed as described in Materials and Methods. ND, not detectable. Average values (±SE) are given.