TABLE 1.
DLS data of the soluble TraB-derived proteins in GFacetate
| Protein (NBD1 and/or NBD2)a | MMCalc (kDa)b | Hexamer MMCalc (kDa)c | MMDLS (kDa)d | Radius (nm)e | Polydispersity (nm [%]) |
|---|---|---|---|---|---|
| TraBFL (WT/WT) | 102.6 | 615.6 | 593.0 | 9.1 | 3.5 (38.1) |
| TraBFL (WT/KA) | 102.6 | 615.6 | 603.4 | 9.2 | 3.1 (33.6) |
| TraBFL (RA/WT) | 102.6 | 615.6 | 666.9 | 9.6 | 3.8 (39.6) |
| TraBFL (RA/KA) | 102.6 | 615.6 | |||
| TraBNT (WT) | 54.6 | 327.6 | 365.6 | 7.4 | 2.9 (39.0) |
| TraBNT (RA) | 54.6 | 327.6 | |||
| TraBCT (WT) | 48.8 | 292.8 | 348.5 | 7.3 | 2.3 (32.4) |
| TraBCT (KA) | 48.8 | 292.8 | 353.3 | 6.9 | 2.3 (24.2) |
a
The presence or absence of nucleotide-binding domain mutation(s) is shown in parentheses for the full-length TraB and fragments. WT, wild type; RA, R53A mutation in NBD1; KA, K504A mutation NBD2.
b
MMCalc, theoretical molecular mass calculated from amino acid sequence.
c
The hexamer molecular mass was calculated as MMCalc × 6.
d
Molecular mass experimentally determined by DLS.
e
Estimation of the hydrodynamic radius of the particle.