TABLE 2.
Summary of kinetic parameters of TraB, TraB fragments, and TraB mutants and comparison with TrwKa
| Protein (NBD1 and/or NBD2)b | Kinetics with Michaelis-Menten equation |
Kinetics with Lineweaver-Burk plotc |
|||
|---|---|---|---|---|---|
| Vmax (nmol min−1 mg−1) | Vmax (mol min−1 mol−1) | Km (mM) | Vmax (nmol min−1 mg−1) | Km (mM) | |
| TraBFL (WT/WT) | 126.2 ± 4.6 | 12.9 ± 0.4 | 0.59 ± 0.07 | 120.5 | 0.52 |
| TraBFL (WT/KA) | 25.0 ± 0.8 | 2.6 ± 0.1 | 0.60 ± 0.09 | 23.9 | 0.54 |
| TraBFL (RA/WT) | 30.1 ± 0.5 | 3.1 ± 0.1 | 0.31 ± 0.02 | 30.03 | 0.30 |
| TraBNT (WT) | 121.8 ± 4.3 | 6.8 ± 0.2 | 0.48 ± 0.07 | 130 | 0.49 |
| TraBCT (WT) | 45.1 ± 1.5 | 2.2 ± 0.1 | 0.62 ± 0.07 | 42.2 | 0.52 |
| TrwK | 48.4 | 0.7 | NA | NA | |
a
Values were determined at 37°C.
b
See Table 1, footnote a, for an explanation of the protein designations.
c
NA, not available.