FIG. 3.

WalK_Spn∼P disappearance due to phosphoryltransfer to added WalR_Spn. A representative reaction progression curve used to determine half-lives in Table 2 is shown. Open symbols, WalK_SpnΔN35 (C)-His∼P decay in the absence of WalR_Spn in reaction mixtures containing Mg²⁺ or Ca²⁺; closed symbols, decay of WalK_SpnΔN35 (C)-His∼P in parallel reaction mixtures containing Mg²⁺ or Ca²⁺ and WalR_Spn added at t = 0. See Materials and Methods for details. These reactions contained 1.5 μM WalK_SpnΔN35 (C)-His∼P and 0.25 μM WalR_Spn in a reaction volume of 100 μl at 25°C.