TABLE 2.
Amino acid | Concn (%)a in culture supernatant |
||
---|---|---|---|
SA113 | Δsdh | Δsdh (pRBsdh) | |
Alanine | 22.0 ± 4.7** | 47.4 ± 2.0** | 33.8 ± 4.0 |
Glutamate | 19.1 ± 6.0** | 43.8 ± 3.3** | 30.0 ± 5.3 |
Histidine | 76.5 ± 0.4* | 90.7 ± 7.1* | 83.9 ± 6.5 |
Isoleucine | 30.4 ± 2.7* | 48.0 ± 5.4* | 40.5 ± 0.7 |
Proline | 29.0 ± 14.6** | 91.4 ± 13.9** | 52.9 ± 12.9 |
Amino acid concentrations in the culture supernatant of cultures grown aerobically in CDM for 24 h. Of the 15 amino acids contained in CDM, the differentially consumed amino acids are listed. An initial amino acid concentration of 1 mM was set as 100%. Values are the averages of three independent experiments ± standard deviations. *, P < 0.1; **, P < 0.05 (Δsdh mutant versus SA113).