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Identification and analysis of monocyte subpopulations. (A) Monocytes were identified and gated based on CD14 staining and side-scatter characteristics. (B) Gated monocytes were subdivided into monocyte subpopulations on the basis of CD14 and CD16 staining characteristics. Subpopulations are defined as CD14+ CD16+ (quadrant 1), CD14++ CD16+ (quadrant 2), CD14++ CD16− (quadrant 3), and CD14+ CD16− (quadrant 4).
