FIG. 3.

Intracellular TNF-α production by different monocyte subpopulations stimulated with IC compared to that of unstimulated, IgG and LPS-treated controls. Results are expressed as mean values ± standard deviations of percentages of monocytes from 24 children (SMA group [n = 9], SMA controls [n = 10], and CM controls [n = 5]) that produced TNF-α. CD14⁺ CD16⁺ monocytes had the highest percentage of TNF-α-positive cells, followed by the CD14⁺⁺ CD16⁺ subset. TNF-α expression by CD14⁺ CD16⁻ and CD14⁺⁺ CD16⁻ monocytes in response to IC stimulation was negligible.