FIG. 2.

Analysis of PDE activity of CdpA in vivo. Intracellular c-di-GMP content in wild-type B. pseudomallei KHW, the cdpA mutant and complemented (Comp.) cdpA mutant. Intracellular nucleotides were extracted from late-stationary-phase bacterial cultures and the c-di-GMP content measured using RP-HPLC. The amount of c-di-GMP was expressed per milligram (wet weight) of cells. Each bar is the mean of three independent experiments and any value which was statistically different (P < 0.05) from the wild type as assessed by the ANOVA test is denoted by an asterisk.