TABLE 1.
Strains, plasmids, and primers used in this study
| Strain, plasmid, or primer | Genotype or sequence | Reference |
|---|---|---|
| Strains | ||
| N. gonorrhoeae 1291 | Wild type | |
| aniA::kan | aniA::kan ΔaniA pGFP | 22 |
| aniA′-′gfp | aniA′-′gfp | This study |
| Plasmids | ||
| pCTS32 | pGEM-T easy/Sptr/ΔproB | 64 |
| pGFP | pLES98/GFP/Chlr | 20 |
| paniA′-′gfp | pCTS32/aniA′-′gfp | This study |
| Primers | ||
| aniA promoter For | 5′-TACCCGGGAACTGCCTTTGCCTGCTCTG-3′ | |
| aniA promoter Rev | 5′-TCTTCTCTTTTACTCATAATGTTTCCTTTT-3′ | |
| GFP For | 5′-AAAAGGAAAACATTATGAGTAAAAGGAGAAGA-3′ | |
| GFP Rev | 5′-GTCTTAAGTTCTGCAGGAGGTCTGGACATT-3′ |
Strains, plasmids, and primers used during this study are listed above with a description of their relevant genotypes or sequences (primers only). The source of each strain or plasmid is also denoted by a citation. Strains and plasmids were constructed during the course of this study. Primers are named for the region they amplify.