TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Collection site or relevant genotype | PFGE typea | Serum growthb | Sourcec or reference |
|---|---|---|---|---|
| A. baumannii | ||||
| 510 | Sputum | A | I | UNMC (NE; 2006) |
| 2824 | Urine | A | UNMC (NE; 2008) | |
| 2898 | Ankle wound | A | UNMC (NE; 2008) | |
| 4564 | Urine | A | UNMC (NE; 2006) | |
| 4860 | Urine | A | UNMC (NE; 2007) | |
| 4884 | Urine | A | UNMC (NE; 2007) | |
| 4893 | Leg wound | A | UNMC (NE; 2007) | |
| 5191 | Urine | A | UNMC (NE; 2007) | |
| 5393 | Unknown | A | UNMC (NE; 2007) | |
| 5562 | Blood | A | I | UNMC (NE; 2007) |
| 5592 | Sputum | A | UNMC (NE; 2007) | |
| 5695 | Unknown | A | UNMC (NE; 2007) | |
| ATCC 17904 | Urine | B | R | ATCC (1963) |
| ATCC 17961 | Blood | C | R | ATCC (1968) |
| ATCC 17976 | Postoperative meningitis | D | I | ATCC (1951) |
| ATCC 17978 | Fetal meningitis | E | I | ATCC (1951) |
| 98-37-01 | Endotracheal tube | F | R | CDC (TX; 1998) |
| 98-37-02 | Sputum | G | R | CDC (TX; 1998) |
| 98-37-05 | Tracheal aspirate | H | I | CDC (TX; 1998) |
| 98-37-09 | Cerebrospinal fluid | I | R | CDC (TX; 1998) |
| ACJ1 | EZ-Tn5::A1S_3365 derivative of 98-37-09 | I | I | This work |
| ACJ2 | EZ-Tn5::A1S_2989 derivative of 98-37-09 | I | I | This work |
| ACJ3 | EZ-Tn5::A1S_0269 derivative of 98-37-09 | I | I | This work |
| ACJ4 | EZ-Tn5::A1S_3045 (promoter region) derivative of 98-37-09 | I | I | This work |
| ACJ5 | EZ-Tn5::A1S_3045 (promoter region) derivative of 98-37-10 | I | I | This work |
| ACJ6 | EZ-Tn5::A1S_3278 derivative of 98-37-09 | I | I | This work |
| 98-37-11 | Bronchial wash | J | R | CDC (TX; 1998) |
| 01-12-05 | Blood | K | R | CDC (IN; 2001) |
| 01-12-06 | Sputum | K | CDC (IN; 2001) | |
| 01-12-07 | Sputum | K | CDC (IN; 2001) | |
| 01-12-08 | Burn wound | K | CDC (IN; 2001) | |
| 01-12-09 | Catheter tip | K | CDC (IN; 2001) | |
| 07-09-54 | Unknown | L | I | CDC (KY; 2007) |
| 07-09-59 | Unknown | L | CDC (KY; 2007) | |
| 07-09-60 | Unknown | L | CDC (KY; 2007) | |
| 07-09-61 | Unknown | L | CDC (KY; 2007) | |
| 07-09-63 | Unknown | L | CDC (KY; 2007) | |
| E. coli | ||||
| INVαF′ | F′ endA1 recA1 hsdR17 (rK− mK+) supE44 thi-1 gyrA96 relA1 ϕlacZΔM15 Δ(lacZYA-argF)U169 λ− | Invitrogen | ||
| Plasmids | ||||
| pCR II-TOPO | Ampr Kanr | Invitrogen | ||
| pWH1266 | Ampr Tetr | 15 | ||
| pACJ01 | pWH1266/A1S_2989 Tetr | This work |
a
PFGE pattern of the isolate in comparison to all other isolates in the study.
b
Human serum growth/resistance profile relative to all other PFGE types assessed; I and R indicate intermediate and robust growth, respectively.
c
Listed are the strain source with the location and/or year of isolation in parentheses. UNMC, University of Nebraska Medical Center.