FIG. 1.
Characterization of the B. abortus Δpgk mutant by Southern blot analysis. EcoRV-digested genomic DNA was probed with the pgk (A), amp (B), or kan (C) DNA fragments. Lanes 1, B. abortus Δpgk mutant; lanes 2, clones in which a single recombination event took place; lanes 3, B. abortus S2308; lanes MW, molecular weight markers.