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Western blot analysis of pgk expression in different B. abortus strains. B. abortus S2308 (lane 1), Δpgk mutant (lane 2), or Δpgk mutant complemented with pBBR1-pgk plasmid (lane 3) were subjected to SDS-PAGE, and Western blot analysis using polyclonal anti-PGK antibodies demonstrated the presence of a band of approximately 42 kDa in lanes 1 and 3, which indicated pgk expression.
