FIG. 5.
Persistence of B. abortus S2308 or Δpgk in BALB/c, C57BL/6, or 129/Sv mice. Eight mice were infected i.p. with a dose of 106 CFU of bacteria. Spleens were harvested at different times, and the number of CFU in disrupted tissue was determined by 10-fold serial dilution and plating. The values are means and standard deviations. The asterisks indicate statistically significant differences between the results obtained for the group that received the B. abortus Δpgk mutant compared to the animals injected with the B. abortus parental strain S2308 (P ≤ 0.05).