TABLE 1.
Bacterial strains and vectors used in this study
| Strain or plasmid | Characteristics | Source or reference |
|---|---|---|
| Strains | ||
| E. coli TOP 10F | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| B. abortus strains | ||
| S2308 | Wild type; smooth; virulent | Laboratory stock |
| S19 | Vaccine strain; smooth | Laboratory stock |
| RB51 | Rifr; rough mutant of S2308 | Laboratory stock |
| Δpgk strain | Kanr; Δpgk mutant of S2308 | This study |
| Plasmids | ||
| pUC4K | ColE1; Ampr Kanr | GE Healthcare |
| pBluescript KS | ColE1; bla | Stratagene |
| pBBR1MCS | Broad-host-range cloning vector; Cmr | 21 |
| pBBR1-pgk | 1,345-kb KpnI and BamHI fragment containing the complete B. abortus S2308 pgk gene cloned into pBBR1MCS | This study |