FIG. 8.

Association of the Y. pseudotuberculosis Δhfq strain with host cells. The Y. pseudotuberculosis wild-type, Δhfq, or Δhfq+phfq strain was incubated with J774 murine macrophage-like cells (MOI of 10). (a) Percentage of inoculum associated with host cells. (b) Percent intracellular bacteria after 30 min of treatment with gentamicin compared to untreated cells and percent intracellular bacteria after 2 and 4 h compared to CFU after 30 min. Bars represent the mean percentages, and error bars are standard errors of CFU from triplicate wells. Statistical analysis was performed with Student's unpaired t test. Data are representative of 3 independent experiments.