FIG. 6.

Increased p47phox expression in leukocytes recovered from WT and 5-LO KO mice treated with aerosolized LTB₄ following S. pneumoniae challenge. WT and 5-LO KO mice were infected with 10⁶ CFU of S. pneumoniae cells via the i.n. route and treated with aerosolized vehicle or LTB₄ (100 ng) 24 h later. (A) Confocal images of cells recovered by lavage 24 h after LTB₄ (48 h postinfection) and immunostained for p47phox are shown. (B) Fluorescence was quantitated in a minimum of 50 cells per condition as described in Materials and Methods. *, P < 0.05 versus results for cells from uninfected WT or 5-LO KO mice, and #, P < 0.05 versus results for cells from infected WT or 5-LO KO mice treated with vehicle, using the Student t test. Data are expressed as the percentages of the uninfected and untreated value, and bars represent the mean results ± standard errors of the means for fluorescence determined from confocal images of cells of 3 to 5 animals per group. (C) The immunoblot of p47phox in BALF cell lysates is from a single experiment that is representative of a total of 3 independent experiments. The numbers under the blots represent the arbitrary densitometric units for each band.