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Concurrent binding of CFH and PLG to BpcA. A competition inhibition assay was performed, adding different amounts of PLG or CFH to inhibit the binding of 20 μg/ml PLG (upper panel) or 0.2 μg/ml CFH (lower panel) to immobilized BpcA. Bound CFH and PLG were detected using goat anti-CFH (dashed line) and goat anti-PLG (solid line), respectively, followed by peroxidase-conjugated secondary antibody.
