FIG. 1.

CCL19, CCL21, and CCR7 are upregulated during the course of T. gondii infection. Infected mice were sacrificed at days 7, 14, 21, and 42 postinfection, and total RNA was extracted from the livers and brains. Real-time RT-PCR was conducted to determine the absolute copy numbers of CCL19, CCL21, and CCR7 using a standard curve, and they were compared to those of the internal reference HPRT gene. The data are represented as mean fold changes over naïve mice, with the error bars representing standard errors of the mean (SEM) of at least 3 biological replicates.