FIG. 2.

Deficiency in proinflammatory cytokine production during acute infection in CCR7^−/− mice. (a) Serum samples were collected from C57BL/6 and CCR7^−/− mice at day 7 postinfection, and the protein levels of IFN-γ, CCL2, IL-12p70, IL-10, IL-6, and TNF-α were measured. The levels (averages ± SEM) for individual mice (n = 3) are plotted. (b) Single-cell suspensions were generated from naïve and infected spleens and restimulated with sTAg. IFN-γ production was measured after 48 h using ELISA. Averages and SEM of at least 3 biological replicates are plotted and are representative of 3 independent experiments containing a minimum of 3 biological replicates. *, P < 0.001.