FIG. 2.

Adoptive transfer of Ag-BMDC to CCR2^−/− mice suppresses IL-4 production. (A) Schematic diagram of adoptive transfer experiments. (B) Log₁₀ relative quantification (RQ) of IL-4 transcription from whole lungs 7 days postinfection compared to uninfected lung expression. Prior to infection BMDC were not treated (Ag-free) or were matured (mat) and exposed to heat-killed H. capsulatum (Ag) or ovalbumin (ova) (n = 6 to 9). (C) Levels of IL-4 protein in whole-lung homogenates as determined by ELISA (n = 6). (D) IL-12 transcription by WT BMDC prior to transfer compared to IL-12 transcription by untreated WT BMDC. **, P = 0.002 compared to CCR2^−/−; *, P < 0.05 compared to CCR2^−/−; #, P < 0.05 compared to Ag-BMDC that were not matured. The data are the means and SEM of two or three experiments.