TABLE 3.
Cross-reactivity of DBL5 sera on lab-adapted parasite lines and clinical isolates
| Serum | Adjusted geometric mean of fluorescence intensity fora: |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CSA-selected lab-adapted parasite line |
Control |
Clinical isolate |
||||||||||
| FCR3 | 7G8 | HB3 A | HB3 B | Pf2004 | NF54 | Pf2006 | A4ultra | ItG-ICAM | 736 1xpcsa | 711 1xpcsa | 755 | |
| Pool of plasma from multigravid Tanzanian females | 489 | 175 | 349 | 212 | 686 | 234 | 377 | 176 | 98 | 381 | 104 | 126 |
| Pool of plasma from multigravid Cameroonian females | 55 | 126 | 323 | 241 | 469 | 120 | 62 | 106 | 507 | 180 | 39 | 79 |
| Pool of plasma from Cameroonian males | 19 | 59 | 137 | 98 | 95 | 60 | 62 | 251 | 1,648 | 94 | 32 | 19 |
| Nonimmune plasma | 11 | 21 | 29 | 11 | 39 | 32 | 14 | 6 | 7 | 86 | 3 | 52 |
| 7G8-DBL1var2csa, rabbit 1 | 15 | 59 | 58 | 6 | 14 | 63 | 11 | 0 | ND | ND | ND | ND |
| 7G8-DBL1var2csa, rabbit 2 | 16 | 22 | 45 | 13 | 77 | 58 | 1 | 0 | ND | ND | ND | ND |
| 7G8-DBL1var2csa, rabbit 3 | 6 | 43 | 36 | 29 | 11 | 14 | 6 | 2 | ND | ND | ND | ND |
| 3D7-DBL5var2csa, rabbit 1 | 28 | 63 | 143 | 37 | 31 | 177 | 16 | 0 | 4 | 184 | 24 | 46 |
| 3D7-DBL5var2csa, rabbit 2 | 106 | 158 | 218 | 232 | 183 | 326 | 97 | 34 | 47 | 414 | 1,143 | 446 |
| 3D7-DBL5var2csa, rabbit 3 | 163 | 104 | 119 | 122 | 39 | 397 | 6 | 2 | 0 | 447 | 0 | 14 |
| 7G8-DBL5var2csa, rabbit 1 | 279 | 422 | 740 | 297 | 140 | 185 | 23 | 3 | 2 | 253 | 188 | 0 |
| 7G8-DBL5var2csa, rabbit 2 | 47 | 189 | 158 | 50 | 110 | 103 | 16 | 8 | 0 | 253 | 137 | 6 |
| 7G8-DBL5var2csa, rabbit 3 | 55 | 213 | 336 | 207 | 208 | 206 | 11 | 11 | 1 | 417 | 264 | 0 |
| IT-DBL5var2csa, rabbit 1 | 493 | 16 | 1,314 | 799 | 170 | 79 | 88 | 5 | 0 | 219 | 37 | 37 |
| IT-DBL5var2csa, rabbit 2 | 743 | 74 | 1,843 | 465 | 107 | 66 | 138 | 1 | 0 | 336 | 12 | 0 |
| IT-DBL5var2csa, rabbit 3 | 659 | 29 | 1,567 | 636 | 220 | 18 | 37 | 2 | 0 | 0 | 3 | 0 |
| IT-DBL3var22, rabbit 1 | 0 | 24 | 0 | 0 | 0 | 4 | 1 | 3 | 0 | 0 | 0 | 0 |
| IT DBL3var22, rabbit 2 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 5 | 0 | 0 | 0 |
| IT-DBL3var22, rabbit 3 | 0 | 0 | 5 | 28 | 0 | 6 | 34 | 0 | 1 | 0 | 12 | 4 |
| A4-CIDR1, rabbit 1 | 0 | 3 | 0 | 3 | 6 | 0 | 15 | 2,300 | 16 | 0 | 0 | 0 |
a
Reactivity is expressed as the adjusted geometric mean of fluorescence intensity. Immune serum reactivities below that of the negative-control IT-DBL3var22 were considered negative. Boldface numbers represent those for parasite lines expressing a homologous PfEMP1 variant. ND, not done.