TABLE 1.
Y. pestis strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Strainsa | ||
| KIM5 | Pgm− (Δpgm; Hms− Ybt−) Lcr+ Pla+; pMT1, pCD1, pPCP1 | 92 |
| KIM5(pCD1Ap)+ | Apr Pgm+ (Hms+ Ybt+) Lcr+ Pla+; pMT1, pCD1Ap (′yadA::bla), pPCP1; derived from KIM6+ | 45 |
| KIM5(pCD1Ap) | Apr Pgm− (Δpgm; Hms− Ybt−) Lcr+ Pla+; pMT1, pCD1Ap (′yadA::bla), pPCP1; derived from KIM6 | This study |
| KIM5-2045.1 (pCD1Ap) | Apr Hms+ Ybt− (Δpsn2045.1) Lcr+ Pla+; pMT1, pCD1Ap (′yadA::bla), pPCP1; derived from KIM6-2045.1 | This study |
| KIM5-2045.6 (pCD1Ap) | Kmr Apr Hms+ Ybt− (Δpsn::kan2045.6) Lcr+ Pla+; pMT1, pCD1Ap (′yadA::bla), pPCP1; derived from KIM6-2045.6 | This study |
| KIM5-2046.1 (pCD1Ap) | Kmr Apr Hms+ Ybt− (irp2::kan2046.1) Lcr+ Pla+; pMT1, pCD1Ap (′yadA::bla), pPCP1; derived from KIM6-2046.1 | This study |
| KIM5-2046.3 (pCD1Ap) | Apr Hms+ Ybt− (Δirp2-2046.3) Lcr+ Pla+; pMT1, pCD1Ap (′yadA::bla), pPCP1; derived from KIM6-2046.3 | This study |
| KIM6+ | Pgm+ (Hms+ Ybt+) Lcr− Pla+; pMT1, pPCP1 | 38 |
| KIM6 | Pgm− (Δpgm; Hms− Ybt−) Lcr− Pla+; pMT1, pPCP1 | 38 |
| KIM6-2045.1 | Hms+ Ybt− (Δpsn2045.1) Lcr− Pla+; pMT1, pPCP1 | 38 |
| KIM6-2045.6 | Kmr Hms+ Ybt− (Δpsn::kan2045.6) Lcr− Pla+; pMT1, pPCP1 | 36 |
| KIM6-2046.1 | Kmr Hms+ Ybt− (irp2::kan2046.1) Lcr− Pla+; pMT1, pPCP1 | 38 |
| KIM6-2046.3 | Hms+ Ybt− (Δirp2-2046.3) Lcr− Pla+; pMT1, pPCP1 | 7 |
| KIM6-2180 | Hms+ Ybt− (Δirp2-2046.3 Δpsn2045.1) Lcr− Pla+; pMT1, pPCP1 | This study |
| Plasmids | ||
| pCD1Ap | 71.7 kb, Apr Lcr+; pCD1 with bla cassette inserted into ′yadA downstream of the frameshift mutation in this pseudogene | 45 |
| pCSIRP498.9 | 8.7 kb, Apr Sucs (sacB+), Δirp2-2046.3, R6K origin suicide vector | 7 |
a
A plus sign indicates an intact chromosomal 102-kb pgm locus. All other Y. pestis strains have a mutation within this locus or a deletion of the entire locus.