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. 2010 Mar 15;78(5):2189–2198. doi: 10.1128/IAI.00021-10

FIG. 1.

FIG. 1.

EMSA. PmrA binding. Mobility shift assays were used to determine if purified proteins bound to regulated gene promoters. (A) His-PmrA binds to the pmrA (12 ng; lanes 1 to 6) and pdpD (12 ng; lanes 7 to 12) promoters in a dose-dependent manner. (B) His-PmrA binding to the pmrA promoter is affected by specific competitor DNA but not nonspecific competitor DNA. Each lane contains 12 ng of labeled pmrA promoter. (C) His-PmrA binding to the pdpD promoter is affected by specific but not nonspecific competitor DNA. Each lane contains 12 ng of labeled pdpD promoter.