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. 2010 Mar 15;78(5):2189–2198. doi: 10.1128/IAI.00021-10

FIG. 7.

FIG. 7.

Coprecipitation. PmrA, MglA, and SspA coprecipitate. Western blot analysis of soluble lysates to which His-PmrA or His-MglA was added and precipitated with His-bind resin. (A) Anti-PmrA sera. (B) Anti-MglA sera. (C) Anti-His monoclonal antibody. Lane 1, His-PmrA added to an F. novicida ΔpmrA soluble fraction and precipitated with His-bind resin; lane 2, His-MglA added to an F. novicida ΔmglA soluble fraction and precipitated with His-bind resin; lane 3, His-SspA from F. novicida with chromosomally encoded His-tagged sspA precipitated with His-bind resin.