TABLE 1.
S. mutans strains used in this study
| Strain designation | Genotype or characteristics | Source or reference |
|---|---|---|
| UA159 | Wild-type ATCC 700610 | ATCC |
| EA72 | UA159 ΔpknB::aphA3 Kmr | 16 |
| EA74 | UA159 ΔpppL::erm Ermr | This study |
| EA75 | UA159 ΔpknB::aphA3 ΔpppL::erm Kmr Emr | This study |
| OMZ 955 | UA159 Δhk11::ermAM | D. Cvitkovitch, University of Toronto |
| OMZ 953 | UA159 ΔvicK::ermAM | D. Cvitkovitch, University of Toronto |
| OMZ 1110 | UA159 ΔciaRH::erm | This study |
| OMZ 1111 | UA159 ΔcomDE::erm | This study |
| OMZ 1112 | UA159 ΔpknB::aphA3 ΔciaRH::erm | This study |
| EA82 | EA72 carrying pLR16T, with 0.7-kb fragment containing pknB plus RBS | This study |
| EA83 | EA74 carrying pLR16T with 1.8-kb fragment containing pppL with RBS | This study |
| EA84 | EA75 carrying pLR16T with 2.6-kb fragment containing pppL and pknB with RBS | This study |
| EA79 | EA72 carrying pDL278 containing Perm from pVA838 and 0.7-kb fragment containing pknB with RBS | This study |
| EA80 | EA74 carrying pDL278 containing Perm from pVA838 and 1.8-kb fragment containing pppL with RBS | This study |
| EA81 | EA75 carrying pDL278 containing Perm from pVA838 and 2.6-kb fragment containing pppL and pknB with RBS | This study |
| EA78 | EA72 carrying pVA838 containing Perm and pknB with RBS | 16 |