TABLE 3.
Transformation efficiencies of wild type and mutantsa
| Strain | Count (CFU/ml) |
Transformation frequency | |
|---|---|---|---|
| Total | Transformants | ||
| UA159 | 5.6 × 109 (1.0 × 109-1.9 × 1010) | 4.4 × 103 (1.8 × 103-5.2 × 103) | 7.8 × 10−7 |
| EA72 | 4.6 × 108 (4.0 × 108-5.3 × 108) | 1.2 × 102 (3.0 × 101-1.6 × 102) | 2.6 × 10−7 |
| EA79 | 7.1 × 108 (6.5 × 108-8.0 × 108) | 4.9 × 103 (1.0 × 103-6.1 × 103) | 6.9 × 10−6 |
| EA74 | 6.0 × 108 (5.5 × 108-7.2 × 108) | 5 (0-20) | 8.3 × 10−9 |
| EA80 | 8.1 × 108 (6.2 × 108-9.0 × 108) | 2.2 × 103 (8.5 × 102-2.6 × 103) | 2.7 × 10−6 |
| EA75 | 4.2 × 108 (4.0 × 108-4.5 × 108) | 5 (0-10) | 1.1 × 10−8 |
| EA81 | 8.6 × 108 (8.0 × 108-9.0 × 108) | 1.5 × 103 (7.3 × 102-2.3 × 103) | 1.7 × 10−6 |
a
Results shown are the median counts (ranges) from three independent experiments, each performed in duplicate. All the mutant strains showed a significant reduction in transformability compared to the parent strain (P = 0.02 for EA72; P = 0.005 for EA74; P = 0.005 for EA75). The complemented strains showed a significant increase in transformability compared to the respective mutant strains (P = 0.005 for all three strains).