TABLE 5.
Up- and downregulated genes in pknB mutant strain EA72
| ORF | Gene | Predicted protein or gene function | Change in expression (fold) |
|---|---|---|---|
| SMU.503c | Hypothetical protein | 4.61 | |
| SMU.984 | Hypothetical protein | 3.95 | |
| SMU.609 | Putative 40-kDa cell wall protein precursor | 3.31 | |
| SMU.1067c | Putative ABC transporter, permease protein | 2.84 | |
| SMU.334 | Argininosuccinate synthase (citrulline-aspartate ligase) | 2.81 | |
| SMU.21 | mreD | Putative cell shape-determining protein | 2.80 |
| SMU.753 | Conserved hypothetical protein | 2.79 | |
| SMU.20 | mreC | Putative cell shape-determining protein | 2.77 |
| SMU.614 | Hypothetical protein | 2.70 | |
| SMU.667 | nrdG | Putative ribonucleotide reductase, small subunit | 2.46 |
| SMU.985 | bglA | Putative β-glucosidase | 2.44 |
| SMU.1086 | kitH | Putative thymidine kinase | 2.44 |
| SMU.815 | Putative amino acid transporter, amino acid-binding protein | 2.43 | |
| SMU.510c | Hypothetical protein | 2.41 | |
| SMU.1422 | pdhB | Putative pyruvate dehydrogenase E1 component β-subunit | 2.37 |
| SMU.663 | argC | Putative N-acetyl-γ-glutamyl-phosphate reductase (N-acetyl-glutamate-γ-semialdehyde dehydrogenase) | 2.33 |
| SMU.1487 | Conserved hypothetical protein | 2.32 | |
| SMU.1424 | pdhD | Putative dihydrolipoamide dehydrogenase | 2.30 |
| SMU.1402c | Conserved hypothetical protein | 2.29 | |
| SMU.335 | Argininosuccinate lyase | 2.22 | |
| SMU.1070c | Conserved hypothetical protein | 2.18 | |
| SMU.877 | agaL | α-Galactosidase | 2.16 |
| SMU.529 | Hypothetical protein | 2.13 | |
| SMU.1404c | Conserved hypothetical protein | 2.07 | |
| SMU.879 | msmF | Multiple sugar-binding ABC transporter, permease protein | 2.05 |
| SMU.673 | Conserved hypothetical protein | 2.03 | |
| SMU.1896c | bmsH | Hypothetical protein | 0.06 |
| SMU.1895c | Hypothetical protein | 0.06 | |
| SMU.2146c | Hypothetical protein | 0.07 | |
| SMU.1882c | Hypothetical protein | 0.19 | |
| SMU.1063 | opuAa | Putative ABC transporter, ATP-binding protein, proline/glycine betaine transport system | 0.20 |
| SMU.1982c | Conserved hypothetical protein | 0.21 | |
| SMU.1981c | Conserved hypothetical protein | 0.21 | |
| SMU.1984 | comYC | Putative competence protein | 0.22 |
| SMU.1980c | Conserved hypothetical protein | 0.23 | |
| SMU.1987 | comYA | Putative ABC transporter, ATP-binding protein; late competence gene | 0.23 |
| SMU.285 | Hypothetical protein | 0.23 | |
| SMU.1914c | bsmA | Hypothetical protein | 0.23 |
| SMU.625 | comEA | Putative competence protein | 0.25 |
| SMU.277 | Hypothetical protein | 0.26 | |
| SMU.910 | gtfD | Glucosyltransferase-S | 0.26 |
| SMU.1912c | Hypothetical protein | 0.28 | |
| SMU.1908c | Hypothetical protein | 0.29 | |
| SMU.616 | Hypothetical protein | 0.30 | |
| SMU.278 | Hypothetical protein | 0.30 | |
| SMU.1062 | opuAb | Putative ABC transporter, proline/glycine betaine permease protein | 0.32 |
| SMU.453 | mraW | S-Adenosyl-methyltransferase | 0.33 |
| SMU.1906c | Hypothetical protein | 0.34 | |
| SMU.2089 | hexB | Putative mismatch repair protein | 0.34 |
| SMU.618 | Hypothetical protein | 0.34 | |
| SMU.1865 | mutY | Putative A/G-specific DNA glycosylase | 0.35 |
| SMU.281 | Hypothetical protein | 0.36 | |
| SMU.1444c | Conserved hypothetical protein | 0.36 | |
| SMU.1956c | Hypothetical protein | 0.37 | |
| SMU.1910c | Hypothetical protein | 0.39 | |
| SMU.1961c | Putative PTS, sugar-specific enzyme IIA component | 0.39 | |
| SMU.1909c | Hypothetical protein | 0.41 | |
| SMU.283 | Hypothetical protein | 0.42 | |
| SMU.2038 | pttB | Putative PTS, trehalose-specific IIABC component | 0.43 |
| SMU.992 | Hypothetical protein | 0.43 | |
| SMU.1979c | Conserved hypothetical protein | 0.44 | |
| SMU.2037 | treA | Putative trehalose-6-phosphate hydrolase | 0.45 |
| SMU.1957 | Putative PTS, mannose-specific IID component | 0.46 | |
| SMU.2116 | opuCa | Putative osmoprotectant amino acid ABC transporter, ATP-binding protein | 0.46 |
| SMU.279 | Hypothetical protein | 0.48 | |
| SMU.499 | Putative late competence protein | 0.48 | |
| SMU.1958c | Putative PTS, mannose-specific IIC component | 0.50 |