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. 2010 Mar 1;78(5):2089–2098. doi: 10.1128/IAI.00811-09

FIG. 1.

FIG. 1.

Western blot analysis of wild-type and mutant pneumococcal strains. Anti-PhtD antibody was used to probe extracts from the wild-type (wt) and PhtABDE− quadruple mutant (Pht−) strains (R36A, D39, 43, 4-CDC, and 2737) as well as PspA− and/or PspC− mutant derivatives of D39. The molecular sizes of the Pht proteins have previously been reported to range from 92 to 115 kDa (2). The positions of the different Pht bands are indicated on the right in the figure, and molecular mass markers are indicated on the left (kDa).