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. 2010 Mar 1;78(5):2079–2088. doi: 10.1128/IAI.01450-09

FIG. 6.

FIG. 6.

Ectopic expression of 3×Flag AnkBΔA1, AnkBΔA2, or AnkBΔA1A2 in HEK293 cells shows altered cellular distribution compared with WT 3×Flag AnkB. Representative confocal images of HEK293 cells transiently transfected with plasmid DNA encoding 3×Flag AnkB, AnkBΔA1, AnkBΔA2, or AnkBΔA1A2 or the BAP control. At 24 h posttransfection, fixed and permeabilized HEK293 cells were labeled with mouse anti-Flag M2 antibody and an Alexa Fluor 488 secondary antibody (green). Nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole) (blue). The white arrowhead indicates localization of 3×Flag AnkB to the cell plasma membrane.