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. 2010 Mar 16;192(10):2604–2612. doi: 10.1128/JB.01678-09

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FIG. 2. — SDS-PAGE of oxaloacetate decarboxylase (ODx) from C. glutamicum after each step of the purification procedure. Lanes M, prestained molecular mass standard; lane 1, crude extract; lane 2, extract after ultracentrifugation; lane 3, pooled fractions with ODx activity after hydrophobic interaction chromatography; lane 4, pooled fractions with ODx activity after anion-exchange chromatography (stepwise elution); lanes 5 and 6, single fractions with ODx activity after size exclusion chromatography; lane 7, single fraction with ODx activity after anion-exchange chromatography (gradient elution).