FIG. 1.
Transcriptional regulation of dmlA-lacZ gene fusion by C4-dicarboxylates and the regulators DmlR, TtdR, and DcuS during aerobic growth. The dmlA-lacZ gene fusion was present on plasmid pMW323, and its expression was measured in E. coli wild-type strain MC4100 and dmlR-, ttdR-, and dcuS-deficient derivatives. Bacteria were grown under aerobic conditions in eM9 medium containing gluconate (50 mM) and the effectors indicated (50 mM). The strains used were strains MC4100 (wild type) (open bars), IMW533 (dmlR) (black bars), IMW523 (ttdR) (gray bars), and IMW262 (dcuS) (striped bars).