TABLE 1.
Strains and plasmids
| Strain/plasmid | Description | Source/reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| S17-1 | recA pro hsdR RP4-2-Tc::MuKm::Tn7 tra+ Kmr Spr | 27 |
| JM109 | recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi (lac-proAB) | New England Biolabs |
| R. sphaeroides | ||
| 2.4.1 | Wild type | 33 |
| 2.4.1(pRK415) | Wild type harboring pRK415, Tcr | This study |
| TF18 | rpoE chrR mutation in 2.4.1, Tpr | 26 |
| TF18(pRK2.4.1rpoEchrR) | TF18 harboring pRK2.4.1rpoEchrR | This study |
| TF18(pRK415) | TF18 harboring pRK415, Tcr | This study |
| 2.4.1ΔrpoHII | 2.4.1rpoHII::ΩSpr/Smr | 22 |
| 2.4.1ΔrpoHII(pRK415) | 2.4.1ΔrpoHII harboring pRK415, Tcr | 26 |
| 2.4.1ΔrpoHII(pRK2.4.1rpoHII) | 2.4.1ΔrpoHI harboring pRK2.4.1rpoHII | 26 |
| 2.4.1ΔrpoHI | 2.4.1rpoHI::Kmr cassette | This study |
| 2.4.1ΔrpoHI(pRK415) | 2.4.1ΔrpoHI harboring pRK415, Tcr | This study |
| 2.4.1ΔrpoHI(pRK2.4.1rpoHI) | 2.4.1ΔrpoHI harboring pRK2.4.1rpoHI | This study |
| 2.4.1ΔrpoHI ΔrpoHII | 2.4.1ΔrpoHII rpoHI::Kmr cassette | This study |
| 2.4.1ΔrpoHI ΔrpoHII(pRK415) | 2.4.1ΔrpoHI ΔrpoHII harboring pRK415, Tcr | This study |
| 2.4.1ΔrpoHI ΔrpoHII (pRK2.4.1rpoHI) | 2.4.1ΔrpoHI ΔrpoHII harboring pRK2.4.1rpoHI | This study |
| TF18ΔrpoHI | TF18 rpoHI::Kmr cassette | This study |
| TF18ΔrpoHI(pRK415) | TF18ΔrpoHI harboring pRK415, Tcr | This study |
| TF18ΔrpoHI(pRK2.4.1rpoHI) | TF18ΔrpoHI harboring pRK2.4.1rpoHI | This study |
| Plasmids | ||
| pPHU281 | Suicide vector for R. sphaeroides, Tcr | 15 |
| pUC4K | Kmr, source of Kmr cassette | 25 |
| pRK415 | Tcr | 18 |
| pPHU2.4.1rpoHI::Kmr | pPHU281 with Kmr cassette, flanked by the upstream and downstream regions of rpoHI | This study |
| pRK2.4.1rpoHI | pRK415 harboring a 1.2-kb fragment containing rpoHI, flanked by the 239-bp upstream and 26-bp downstream regions | This study |
| pRK2.4.1rpoEchrR | pRK415 harboring a 1.6-kb fragment containing the rpoE-chrR operon, flanked by the 241-bp upstream and 158-bp downstream regions | This study |
| pDrive cloning vector | Apr Kmr | Qiagen |