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. 2010 Mar 16;192(10):2535–2545. doi: 10.1128/JB.01689-09

TABLE 5.

Intergeneric gordonii-cAM373-induced plasmid transfer

Donora Donor inductionb Recipient Filter mating time Selection Frequencyc Cotransfer of unselected marker (%)d
JH2-2/pAM378/pAMS470 No OG1X 4 h Em 7.8 × 100 30
Tc 4.4 × 100 86
JH2-2/pAMS470 No OG1X 4 h Em < 3.0 × 10−8 NA
JH2-2/pAM378/pAMS470 No CH1S 30 min Em 3.8 × 10−7 ND
Tc 1.1 × 10−5 ND
JH2-2/pAM378/pAMS470 Yes CH1S 30 min Em 7.0 × 10−4 0
Tc 1.1 × 10−4 7
JH2-2/pAM378/pAMS749-12 No CH1S 30 min Em < 3.6 × 10−8 NA
Tc 4.8 × 10−6 0
JH2-2/pAM378/pAMS749-12 Yes CH1S 30 min Em < 4.3 × 10−8 NA
Tc 7.3 × 10−6 0
JH2-2/pAMS470 No CH1S 4 h Em < 6.5 × 10−8 NA
JH2-2/pAM378 No CH1S 4 h Tc 1.2 × 10−5 NA
a

pAM378 is a pAM373::Tn918 Tc-resistant derivative responsive to pheromone. pAMS749-12 is a cloning vector carrying Em resistance. pAMS470 is pAMS749-12 carrying oriT373.

b

Induction involved 90-min exposure of the donor to 50 ng/ml of the synthetic peptide gordonii-cAM373 before donor and recipient were mixed.

c

Conjugation frequencies are given as transconjugants per donor. Frequencies of conjugation to recipient CH1S are mean averages of 3 independent experiments. High rates for pheromone-induced conjugative transfer for positive-control E. faecalis recipients are consistent with published results (11).

d

To determine the cotransfer rate, 200 transconjugants in each case were screened for resistance to the unselected marker (Em or Tc). NA, not applicable; ND, not determined.