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. 2010 Feb 22;30(9):2120–2134. doi: 10.1128/MCB.01511-09

FIG. 2.

FIG. 2.

PRH represses VSP genes in conjunction with TLE proteins. (A) Vegf mRNA levels in K562 cells 48 h after transfection with 10 μg pMUG1 (−) or pMUG1 vectors expressing PRH (wild type [WT], PRH F32E, PRH L23A,L24A, or PRH N187A). mRNA levels were determined as for Fig. 1. Values are means and SD (n = 3). (B) As for panel A except that relative levels of expression of Vegfr-1 are shown. Values are means and SD (n = 3). (C) Western blot of Myc-PRH expression in K562 cells 48 h after transfection with the vectors described for panel A. Whole-cell extracts were run on a sodium dodecyl sulfate-polyacrylamide gel (SDS-PAG) and stained with Myc and lamin A/C antibodies. (D) Vegf mRNA levels in K562 shRNA (sh) control cells and PRH shRNA cells 48 h after transfection with pMUG1-MycPRH (5 μg), pCMV2-TLE1(2.5 μg), or both plasmids were determined as for Fig. 1. Values are means and SD (n = 3). (E) As for panel D except that relative levels of expression of Vegfr-1 are shown. Values are means and SD (n = 3).