FIG. 1.

CEP55 is a component of the intercellular bridge. (A) Immunofluorescence was performed using custom-generated goat anti-mouse TEX14 and guinea pig anti-mouse CEP55 antibodies on isolated intercellular bridge preparations from an 8-week-old testis. The results from double staining (a to c), single staining (d and e), and preimmune using goat serum (f) or guinea pig serum (g) are shown as red, TEX14 or goat serum; green, CEP55 or guinea pig serum; and yellow, merged. (B) Western blot analysis of intercellular bridge enrichment using the anti-TEX14 and anti-CEP55 antibodies. (C) The 24 CEP55 peptides that were identified by proteomic analysis. The identified peptides are highlighted in yellow and green, with shorter overlapping peptides underlined.